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5 LakewoodFt. ПG49A GОs G42V GОi1 S54N GОs R201X GОi1, GОs G202T GОo G226L GОs Q227L GОs, GОi1 D273N GОo A366S GОs GОi1 T325A GОt V328A F332A P-loop P-loop P-loop; Mg2 ligand Switch I Catalytic R Switch II Switch II Catalytic Q Switch II Switch II NKXD motif О6-О5 loop О5 (C-terminal helix) Reduced GTPase rate Reduced GTPase rate Weak Mg2 binding; reduced receptor day trading useing point figure charting negative dominant в [72] GОi1, 1ASO, [73] 1AS2, 1AS3 alll [74] в [75,76] Dominant negative в Reduced GTPase rate; activating mutant Reduced rate of GОО release (prevents activation), loss of 1GIT, Mg2 affinity GОi1(G203A)О1О2 Abolishes GTPase activity Switches nucleotide specificity to XTP (in background of Q205L) Increases GDP release rate, decreases thermostability 1BH2 Increases GDP release GОi1(G203A), [77,78] 1GG2 GОi1(Q204L), [72,79] 1GIL в [80] GОi1(A326S), [63,64] в [62] ппппFigure Jokkes Schematic alll GОsвMg2вGTPОS bound to the catalytic domains of adenylyl cyclase.

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